牛结核病（Bovine Tuberculosis）是由牛型结核分枝杆菌（Mycobacterium bovis）引起的一种人兽共患的慢性传染病，我国将其列为二类动物疫病。
为了预防、控制和净化牛结核病，根据《中华人民共和国动物防疫法》及有关的法律法规，特制定本规范。
1 适用范围
本规范规定了牛结核病的诊断、疫情报告、疫情处理、防治措施、控制和净化标准. 本规范适用于中华人民共和国境内从事饲养、生产、经营牛及其产品，以及从事相关动物防疫活动的单位和个人。
2 诊断 2。1 流行特点
本病奶牛最易感,其次为水牛、黄牛、牦牛。人也可被感染。结核病病牛是本病的主要传染源。牛型结核分枝杆菌随鼻汁、痰液、粪便和乳汁等排出体外，健康牛可通过被污染的空气、饲料、饮水等经呼吸道、消化道等途径感染.
2.2 临床特征：
潜伏期一般为3～6周,有的可长达数月或数年。
临床通常呈慢性经过,以肺结核、乳房结核和肠结核最为常见.
肺结核：以长期顽固性干咳为特征，且以清晨最为明显。患畜容易疲劳，逐渐消瘦，病情严重者可见呼吸困难.
乳房结核：一般先是乳房淋巴结肿大，继而后方乳腺区发生局限性或弥漫性硬结,硬结无热无痛，表面凹凸不平。泌乳量下降，乳汁变稀，严重时乳腺萎缩，泌乳停止。 肠结核：消瘦，持续下痢与便秘交替出现，粪便常带血或脓汁。 2.3 病理变化:
在肺脏、乳房和胃肠粘膜等处形成特异性白色或黄白色结节。结节大小不一，切面干酪样坏死或钙化，有时坏死组织溶解和软化，排出后形成空洞。胸膜和肺膜可发生密集的结核结节，形如珍珠状.
2。4 实验室诊断 2.4.1 病原学诊断
采集病牛的病灶、痰、尿、粪便、乳及其它分泌物样品，作抹片或集菌处理（见附件）后抹片,用抗酸染色法染色镜检，并进行病原分离培养和动物接种等试验。
2。4。2 免疫学试验
牛型结核分枝杆菌PPD(提纯蛋白衍生物)皮内变态反应试验（即牛提纯结核菌素皮内变态反应试验)（见GB/T 18646）。
2。5 结果判定
本病依据流行病学特点、临床特征、病理变化可做出初步诊断.确诊需进一步做病原学诊断或免疫学诊断。
2。5。1 分离出结核分枝杆菌(包括牛结核分枝杆菌、结核分枝杆菌)判为结核病牛。 2。5。2 牛型结核分枝杆菌PPD皮内变态反应试验阳性的牛，判为结核病牛. 3 疫情报告
3。1 任何单位和个人发现疑似病牛,应当及时向当地动物防疫监督机构报告.
3.2 动物防疫监督机构接到疫情报告并确认后，按《动物疫情报告管理办法》及有关规定及时上报。
4 疫情处理
4.1 发现疑似疫情，畜主应限制动物移动;对疑似患病动物应立即隔离。
4。2 动物防疫监督机构要及时派员到现场进行调查核实,开展实验室诊断。确诊后，当地人民政府组织有关部门按下列要求处理：
4.2。1 扑杀
对患病动物全部扑杀。 4。2.2 隔离
对受威胁的畜群（病畜的同群畜）实施隔离，可采用圈养和固定草场放牧两种方式隔离。
隔离饲养用草场，不要靠近交通要道，居民点或人畜密集的地区。场地周围最好有自然屏障或人工栅栏。
对隔离畜群的结核病净化，按本规范5。5规定进行。 4.2。3 无害化处理
病死和扑杀的病畜，要按照GB16548-1996《畜禽病害肉尸及其产品无害化处理规程》进行无害化处理.
4。2。4 流行病学调查及检测
开展流行病学调查和疫源追踪;对同群动物进行检测。 4.2。5消毒
对病畜和阳性畜污染的场所、用具、物品进行严格消毒。
饲养场的金属设施、设备可采取火焰、熏蒸等方式消毒；养畜场的圈舍、场地、车辆等，可选用2％烧碱等有效消毒药消毒；饲养场的饲料、垫料可采取深埋发酵处理或焚烧处理；粪便采取堆积密封发酵方式,以及其它相应的有效消毒方式。
4。2。6 发生重大牛结核病疫情时，当地县级以上人民政府应按照《重大动物疫情应急条例》有关规定，采取相应的疫情扑灭措施。
5 预防与控制
采取以“监测、检疫、扑杀和消毒”相结合的综合性防治措施。
5。1 监测 监测对象：牛
监测比例为：种牛、奶牛100％，规模场肉牛10%，其它牛5％，疑似病牛100％.如在牛结核病净化群中(包括犊牛群)检出阳性牛时,应及时扑杀阳性牛，其它牛按假定健康群处理。
成年牛净化群每年春秋两季用牛型结核分枝杆菌PPD皮内变态反应试验各进行一次监测。初生犊牛,应于20日龄时进行第一次监测。并按规定使用和填写监测结果报告，及时上报。
5.2 检疫
异地调运的动物,必须来自于非疫区，凭当地动物防疫监督机构出具的检疫合格证明调运.
动物防疫监督机构应对调运的种用、乳用、役用动物进行实验室检测。检测合格后，方可出具检疫合格证明。调入后应隔离饲养30天，经当地动物防疫监督机构检疫合格后，方可解除隔离。
5。3人员防护
饲养人员每年要定期进行健康检查。发现患有结核病的应调离岗位，及时治疗. 5。4 防疫监督
结核病监测合格应为奶牛场、种畜场《动物防疫合格证》发放或审验的必备条件。动物防疫监督机构要对辖区内奶牛场、种畜场的检疫净化情况监督检查.
鲜奶收购点（站)必须凭奶牛健康证明收购鲜奶。 5。5 净化措施
被确诊为结核病牛的牛群（场）为牛结核病污染群（场），应全部实施牛结核病净化。 5。5.1 牛结核病净化群(场）的建立
5。5.1。1 污染牛群的处理：应用牛型结核分枝杆菌PPD皮内变态反应试验对该牛群
进行反复监测，每次间隔3个月，发现阳性牛及时扑杀，并按照本规范4规定处理.
5.5.1。2犊牛应于20日龄时进行第一次监测，100～120日龄时，进行第二次监测。凡连续两次以上监测结果均为阴性者，可认为是牛结核病净化群。
5。5.1.3凡牛型结核分枝杆菌PPD皮内变态反应试验疑似反应者，于42天后进行复检，复检结果为阳性，则按阳性牛处理; 若仍呈疑似反应则间隔42天再复检一次，结果仍为可疑反应者，视同阳性牛处理。
5.5.2 隔离
疑似结核病牛或牛型结核分枝杆菌PPD皮内变态反应试验可疑畜须隔离复检. 5。5。3 消毒
5.5.3。1 临时消毒：奶牛群中检出并剔出结核病牛后，牛舍、用具及运动场所等按照4。2。5规定进行紧急处理。
5。5。3.2 经常性消毒：饲养场及牛舍出入口处,应设置消毒池,内置有效消毒剂，如3～5％来苏尔溶液或20%石灰乳等。消毒药要定期更换,以保证一定的药效.牛舍内的一切用具应定期消毒；产房每周进行一次大消毒，分娩室在临产牛生产前及分娩后各进行一次消毒。
附件 样 品 集 菌 方 法
痰液或乳汁等样品，由于含菌量较少，如直接涂片镜检往往是阴性结果.此外，在培养或作动物试验时,常因污染杂菌生长较快，使病原结核分枝杆菌被抑制。下列几种消化浓缩方法可使检验标本中蛋白质溶解、杀灭污染杂菌，而结核分枝杆菌因有蜡质外膜而不死亡，并得到浓缩.
1 硫酸消化法
用4－6％硫酸溶液将痰、尿、粪或病灶组织等按1：5之比例加入混合,然后置37℃作用1－2小时，经3000－4000rpm离心30分钟，弃上清，取沉淀物涂片镜检、培养和接种动物。也可用硫酸消化浓缩后,在沉淀物中加入3％氢氧化钠中和，然后抹片镜检、培养和接种动物。
2 氢氧化钠消化法
取氢氧化钠35－40g，钾明矾2g,溴麝香草酚兰20mg（预先用60％酒精配制成0.4％浓度，应用时按比例加入），蒸馏水1000mL混合，即为氢氧化钠消化液.
将被检的痰、尿、粪便或病灶组织按1:5的比例加入氢氧化钠消化液中，混匀后,37℃作用2－3小时，然后无菌滴加5－10％盐酸溶液进行中和，使标本的pH调到6。8左右(此时显淡黄绿色)，以3000－4000rpm离心15－20分钟，弃上清，取沉淀物涂片镜检、培养和接种动物。
在病料中加入等量的4％氢氧化钠溶液，充分振摇5－10分钟，然后用3000rpm离心15－20分钟，弃上清，加1滴酚红指示剂于沉淀物中，用2N盐酸中和至淡红色，然后取沉淀物涂片镜检、培养和接种动物。
在痰液或小脓块中加入等量的1%氢氧化钠溶液,充分振摇15分钟，然后用3000rpm离心30分钟，取沉淀物涂片镜检、培养和接种动物。
对痰液的消化浓缩也可采用以下较温和的处理方法：取1N（或4％）氢氧化钠水溶
液50mL，0。1mol/L柠檬酸钠50mL，N－乙酰－L－半胱氨酸0。5g，混合.取痰一份，加上述溶液2份，作用24－48小时，以3000rpm离心15分钟，取沉淀物涂片镜检、培养和接种动物.
3 安替福民(Antiformin)沉淀浓缩法
溶液A：碳酸钠12g、漂白粉8g、蒸馏水80mL。 溶液B：氢氧化钠15g、蒸馏水85mL。
应用时A、B两液等量混合，再用蒸馏水稀释成15－20％后使用,该溶液须存放于棕色瓶内。
将被检样品置于试管中，加入3－4倍量的15－20%安替福民溶液,充分摇匀后37℃作用1小时，加1－2倍量的灭菌蒸馏水,摇匀，3000－4000rpm离心20－30分钟，弃上清沉淀物加蒸馏水恢复原量后再离心一次，取沉淀物涂片镜检、培养和接种动物。
Prescribed and alternative diagnostic tests for OIE listed diseases
Terrestrial Code Terrestrial Manual Disease Chapter No。 Chapter No. name 11.7。
2.4.7。 Prescribed tests Alternative tests Bovine tuberculosis Tuberculin test Gamma interferon test Country or zone free from bovine tuberculosis
To qualify as free from bovine tuberculosis， a country or zone should satisfy the following requirements:
1. M。 bovis infection in domestic （permanently captive and owned free—range) bovines
including cattle, water buffalo and wood bison is a notifiable disease in the country；
2. an on-going awareness programme should be in place to encourage reporting of all cases
suggestive of bovine tuberculosis;
3. regular and periodic testing of all cattle， water buffalo， and wood bison herds demonstrated
that M。 bovis infection was not present in at least 99。8％ of the herds and 99.9% of the cattle, water buffalo and wood bison in the country or zone for 3 consecutive years； 4. a surveillance programme should be in place to detect bovine tuberculosis in the country or zone through ante-mortem and post—mortem inspection as described in Chapter 6.2.； 5. if the surveillance programme described in points 3 and 4 above has not detected infection
with M。 bovis for 5 consecutive years， surveillance may be maintained through ante—mortem and post—mortem inspection as described in Chapter6。2.；
6. cattle， water buffalo and wood bison introduced into a country or zone free from bovine tuberculosis should be accompanied by a certificate from an Official Veterinarian attesting that they come from a country, zone， compartment or herd free from bovine tuberculosis or comply with the relevant provisions in Article 11。7。5. or in Article 11.7。6。
CHAPTER 11.7.
BOVINE TUBERCULOSIS
Article 11。7。1.
General provisions
The recommendations in this chapter are intended to manage the human and animal health risks associated with Mycobacterium bovis (M. bovis) infection in domestic (permanently captive and owned free-range) bovines including cattle （Bos taurus， B。 indicus and B。 grunniens）, water buffaloes (Bubalus bubalis） and wood bisons (Bison bison and B. bonasus）。 Standards for diagnostic tests are described in the Terrestrial Manual。
Article 11.7。2.
Country or zone free from bovine tuberculosis
To qualify as free from bovine tuberculosis， a country or zone should satisfy the following requirements:
1. M. bovis infection in domestic (permanently captive and owned free—range） bovines
including cattle， water buffalo and wood bison is a notifiable disease in the country; 2. an on-going awareness programme should be in place to encourage reporting of all cases
suggestive of bovine tuberculosis;
3. regular and periodic testing of all cattle, water buffalo， and wood bison herds
demonstrated that M. bovis infection was not present in at least 99.8％ of the herds and 99。9% of the cattle, water buffalo and wood bison in the country or zone for 3 consecutive years;
4. a surveillance programme should be in place to detect bovine tuberculosis in the country or
zone through ante—mortem and post—mortem inspection as described in Chapter 6.2.; 5. if the surveillance programme described in points 3 and 4 above has not detected infection
with M. bovis for 5 consecutive years, surveillance may be maintained through ante—mortem and post—mortem inspection as described in Chapter6。2。;
6. cattle， water buffalo and wood bison introduced into a country or zone free from bovine
tuberculosis should be accompanied by a certificate from an Official Veterinarian attesting that they come from a country， zone， compartment or herd free from bovine tuberculosis or comply with the relevant provisions in Article 11。7。5。 or in Article 11.7.6.
Article 11.7。3.
Compartment free from bovine tuberculosis
To qualify as a compartment free from bovine tuberculosis， cattle， water buffalo or wood bison in a compartment should be certified by the Veterinary Authority as satisfying the following requirements：
1. cattle， water buffalo and wood bison：
a. showed no sign of bovine tuberculosis or lesions at ante—mortem or post-mortem
inspection for at least 3 consecutive years；
b. were over 6 weeks of age at the time of the first test and have shown a negative result
to at least two tuberculin tests carried out at an interval of a minimum of 6 months, the first test being performed at least 6 months following the slaughter of the last affected animal;
c. met one of the following conditions:
i. showed a negative result to a biannual tuberculin test to ensure the
continuing absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is more than 1% of all herds in the country or zone during the last 2 years; or ii. showed a negative result to an annual tuberculin test to ensure the continuing
absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is more than 0。2% but not more than 1% of all herds in the country or zone during the last 2 years; or iii. showed a negative result to a tuberculin test every 3 years to ensure the
continuing absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is not more than 0。2% of all herds in the country or zone during the last 4 years; or iv. showed a negative result to a tuberculin test every 4 years to ensure the
continuing absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is not more than 0。1% of all herds in the country or zone during the last 6 years；
2. cattle， water buffalo and wood bison introduced into the compartment come from a herd
free from bovine tuberculosis。 This condition may be waived for animals which have been isolated for at least 90 days and which， prior to entry into the compartment, were subjected to at least two tuberculin tests carried out at a 6—month interval with negative results with the second tuberculin test performed during the 30 days prior to entry into the compartment；
3. cattle, water buffalo and wood bison in a compartment free from bovine tuberculosis are
protected from contact with wildlife reservoirs of bovine tuberculosis and are managed under a common biosecurity plan protecting them from contamination with M. bovis， and the compartment has been approved by the Veterinary Authority in accordance with Chapters 4.3。 and 4。4。
Article 11.7。4.
Herd free from bovine tuberculosis
To qualify as free from bovine tuberculosis， a herd of cattle， water buffalo， or wood bisons should satisfy the following requirements：
1. the herd is in a country, zone or compartment free from bovine tuberculosis and is certified free by the Veterinary Authority； or
2. cattle， water buffalo and wood bison in the herd：
a. showed no signs of bovine tuberculosis or lesions at ante-mortem or post—mortem
inspection for at least 3 consecutive years；
b. were over 6 weeks of age at the time of the first test and have shown a negative result
to at least two tuberculin tests carried out at an interval of a minimum of 6 months, the first test being performed at least 6 months following the slaughter of the last affected animal；
c. met one of the following conditions:
i. showed a negative result to an annual tuberculin test to ensure the continuing
absence of bovine tuberculosis； or
ii. showed a negative result to a tuberculin test every 2 years to ensure the
continuing absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is not more than 1％ of all herds in the country or zone during the last 2 years； or
iii. showed a negative result to a tuberculin test every 3 years to ensure the
continuing absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is not more than 0.2％ of all herds in the country or zone during the last 4 years; or iv. showed a negative result to a tuberculin test every 4 years to ensure the
continuing absence of bovine tuberculosis if the annual percentage of herds confirmed as infected with tuberculosis is not more than 0.1% of all herds in the country or zone during the last 6 years; 3. cattle, water buffalo and wood bison introduced into the herd come from a herd free from bovine tuberculosis. This condition may be waived for animals which have been isolated for at least 90 days and which， prior to entry into the herd, were subjected to at least two tuberculin tests carried out at a 6—month interval with negative results。
Article 11。7。5.
Recommendations for the importation of cattle, water buffalo and wood bison for breeding or rearing
Veterinary Authorities of importing countries should require the presentation of an international veterinary certificate attesting that the animals：
1. showed no signs of bovine tuberculosis on the day of shipment;
2. originate from a herd free from bovine tuberculosis that is in a country， zone or
compartment free from bovine tuberculosis; or
3. were subjected to the tuberculin test for bovine tuberculosis with negative results during the
30 days prior to shipment and come from a herd free from bovine tuberculosis; or
4. have been isolated for at least 90 days prior to entry into the herd including protection from contact with wildlife reservoirs of bovine tuberculosis and were subjected to at least two tuberculin tests carried out at a six-month interval with negative results with the second tuberculin test performed during the 30 days prior to entry into the herd.
Article 11。7。6.
Recommendations for the importation of cattle, water buffalo and wood bison for slaughter
Veterinary Authorities of importing countries should require the presentation of an international veterinary certificate attesting that the animals：
1. showed no signs of bovine tuberculosis on the day of shipment;
2. originated from a herd free from bovine tuberculosis or were subjected to a tuberculin test
for bovine tuberculosis with negative results during the 30 days prior to shipment； 3. were not being eliminated as part of an eradication programme against bovine tuberculosis.
Article 11.7.7。
Recommendations for the importation of semen of cattle， water buffalo and wood bison
Veterinary Authorities of importing countries should require the presentation of an international veterinary certificate attesting that:
1. the donor animals showed no signs of bovine tuberculosis on the day of collection of the
semen and either：
a. were kept in an artificial insemination centre free from bovine tuberculosis in a country，
zone or compartment free from bovine tuberculosis and which only accepts animals from free herds in a free country, zone or compartment； or
b. showed negative results to tuberculin tests carried out annually and were kept in a
herd free from bovine tuberculosis;
2. the semen was collected, processed and stored in conformity with the provisions of
Chapter 4。5. and Chapter 4。6。
Article 11。7。8.
Recommendations for the importation of embryos/ova of cattle， water buffalo and wood bison
Veterinary Authorities of importing countries should require the presentation of an international veterinary certificate attesting that:
1. the donor females and all other susceptible animals in the herd of origin showed no signs of
bovine tuberculosis during the 24 hours prior to embryo collection； and either
a. originated from a herd free from bovine tuberculosis in a country, zone or compartment
free from bovine tuberculosis； or
b. were kept in a herd free from bovine tuberculosis， and were subjected to a tuberculin
test for bovine tuberculosis with negative results during an isolation period of 30 days in the establishment of origin prior to collection；
2. the embryos/ova were collected, processed and stored in conformity with the provisions of
Chapters 4.7., 4。8. and 4。9。, as relevant。
Article 11。7.9。
Recommendations for the importation of fresh meat and meat products of cattle， water buffalo， and wood bison
Veterinary Authorities of importing countries should require the presentation of an international veterinary certificate attesting that the entire consignment of meat comes from animals which have been subjected to ante—mortem and post-mortem inspections as described in Chapter 6.2。
Article 11。7。10.
Recommendations for the importation of milk and milk products of cattle, water buffalo and wood bison
Veterinary Authorities of importing countries should require the presentation of an international veterinary certificate attesting that the consignment:
1. has been derived from animals in a herd free from bovine tuberculosis； or 2. was subjected to pasteurization; or
3. was subjected to a combination of control measures with equivalent performance as
described in the Codex Alimentarius Code of Hygienic Practice for Milk and Milk Products.
第11.6.
牛结核病
第11.6.1。
一般规定
在本章的建议是为了管理的人类和动物健康相关的风险，牛分枝杆菌分枝杆菌感染（永久圈养和国有散养）在国内牛，包括牛（牛,B。蜓和B.牦牛)，水牛（水牛）和木野牛（野牛野牛B. bonasus）的。 诊断测试的标准描述在陆地手册。
第11.6.2。
牛结核病的国家或区域
牛结核病，一个国家或区域有资格作为应符合下列要求:
1. 2. 3. 4. 5. 牛分枝杆菌 感染，在国内（永久圈养和散养国有）牛包括黄牛，水牛和木野牛是在该国须呈报的疾病 ； 持续的宣传方案要到位，以鼓励所有报告暗示牛结核病的案件 ; 所有的牛 ，水牛和木野牛经常和定期检测牛群表明研究 杆菌 感染是目前至少有99。8％的牛群和99.9％的牛,水牛和木野牛国家或区域连续三年; 一个监视程序要到位。检测牛结核病，在经过宰前和宰后检验的国家或区域在第一章描述 6。2 ; 如果监察计划分3和第4段中所述证明，M。杆菌 感染是目前至少有99.8％的牛群和99。9％的牛，水牛和木野牛国家或区域连续五年,监视可能会维持经过宰前和宰后检验中所述第 6。2章 ； 6. 牛，水水牛和木野牛引入到一个国家或区牛结核病，应伴随着从一个证书证明，他们从一个国家的官方兽医，区，车厢或无牛结核病的牛群，或遵守有关规定在第 11.6。5 或11。6。6条 。
第11。6。3。
无隔间牛结核病 有资格作为一个车厢，牛结核病，牛，水牛或在木野牛舱应当由兽医管理局认证，符合下列要求： 1. a. b.
黄牛,水牛和木野牛：
至少连续三年没有在事前或宰后检验表明牛结核病或病变的迹象;
在第一次测试的时间超过6周龄，并已显示出负面的结果至少有两个结核菌素最少六个月的时间间隔进行测试，第一次测试正在执
行至少6个月以下的虐杀最后受影响的 动物 ； c.
i. 符合下列条件之一：
每年两次结核菌素试验呈阴性结果，以确保牛结核病的持续的情况下，如果牛群证实感染肺结核的年度百分比超过1%的牛群,在过去两年，在国家或区域 ; ii. 呈阴性结果以每年结核菌素试验，以确保持续的情况下，如果牛结核病的年度百分比牛群证实感染肺结核是0.2％以上,但不超过1％的牛群在国家或区域在过去两年； iii. 显示了一个结核菌素试验阴性结果每三年，以确保持续的情况下，牛结核病，如果牛群证实感染肺结核的年度百分比不超过0.2%的牛群在过去四年在全国或区域 ;或 iv. 显示了一个结核菌素试验阴性结果每四年,以确保持续的情况下，牛结核病，如果不超过0。1％的牛群在国家或区域在过去六年的年度百分比牛群证实感染肺结核; 2. 牛，水牛和木野牛将引入车厢来自畜群无牛结核病.这种情况可能会被免除动物已至少90天之前，将进入隔离舱,受到至少两负的成绩在6个月的时间间隔进行了第二结核菌素试验测试结核菌素执行过程中的前30天将进入车厢 ; 3. 黄牛，水牛，并在仓木野牛牛结核病是由接触保护 水库的野生动物，牛结核病，在一个共同的管理，保护与污染他们研究的生物安全计划牛， 车厢内已批准由兽医局与章节按照 4。3 和4.4.
第11。6。4。
无牛结核病的羊群
资格作为一群牛结核病，牛,水牛或木野牛应符合下列要求:
1. 2. a. b.
牛群是在一个国家，区域或车厢无牛结核病和认证由自由兽医局 ； 黄牛，水牛和木野牛畜群： 无牛结核病或病变的迹象表明在事前或宰后检验，至少一年；
在第一次测试的时间超过6周龄,并已显示出负面的结果,至少有两个结核菌素在六个月的最小间隔进行测试; 爆发后恢复自由状态的
情况下,第一次测试进行至少6个月后，最后受影响的动物屠宰 ;
c.
i. ii. 保持空闲状态，符合下列条件之一：
显示一个年度的结核菌素试验阴性结果，以确保持续的情况下，牛结核病; 显示了一个结核菌素试验阴性结果每两年一次，以确保持续的情况下，牛结核病，如果不超过1％的牛群在国家或区域在过去两年的年度百分比牛群证实感染肺结核;或 iii. 显示了一个结核菌素试验阴性结果每三年，以确保持续的情况下，牛结核病，如果牛群证实感染肺结核的年度百分比不超过0.2％的牛群在过去四年在全国或区域 ；或 iv. 显示了一个结核菌素试验阴性结果每四年，以确保持续的情况下，牛结核病，如果不超过0.1％的牛群在国家或区域在过去六年的年度百分比牛群证实感染肺结核; 3. 黄牛,水牛和木野牛引进牛群到来自无牛结核病畜群.这种情况可能放弃已至少90天，进入入口前的动物隔离猪群，受到至少两负的成绩在6个月的时间间隔进行了第二结核菌素试验测试结核菌素执行过程中的前30天将进入羊群。
第11.6。5。
繁育或饲养的牛,水牛和木野牛进口的建议 进口国兽医主管部门应要求介绍了一个国际兽医证书证明的动物： 1. 2. 3. 4. 没有表现出牛结核病日装运标志; 源于畜群无牛结核病是一个国家,区域或隔间无牛结核病; 受到负的成绩在30天的牛结核病的结核菌素试验在装运前，从一个来牛群牛结核病； 已隔离至少90天前进入到畜群包括从保护水库的野生动物，牛结核病接触，并受到至少两个结核菌素进行了负的成绩在6个月的时间间隔进行的第二次结核菌素测试的测试在30天前将进入羊群。
第11.6.6。
进口牛,水牛和木野牛屠宰的建议 进口国兽医主管部门应要求介绍了一个国际兽医证书证明的动物： 1. 2. 3.
没有表现出牛结核病日装运标志;
源于牛结核病畜群或装运前进行结核菌素试验为负的成绩在30天的牛结核病; 没有被淘汰，对牛结核病根除计划的一部分.
第11.6。7。
进口牛,水牛和木野牛精液的建议 进口国兽医主管部门应当要求国际兽医证书证明,一个演示： 1. a. b. 2.
捐助动物当天收集的精液没有牛结核病的迹象表明,要么： 被保存在人工授精中心，从牛结核病,牛结核病,其中仅接受免费动物免费牛群在一个自由的国家，区域或隔间在国家，区域或车厢 ; 每年进行结核菌素试验，结果均为阴性,在保持牛群牛结核病; 精液收集，处理和储存符合4.5章节的规定 。和 4.6。
第11。6。8。
进口胚胎/牛的卵子，水牛和木野牛的建议 进口国兽医主管部门应当要求国际兽医证书证明，一个演示: 1. a. b. 2.
捐助的女性和所有其他易感动物在畜群起源没有牛结核病的迹象表明，在24小时前胚胎收集，要么 源自畜群在一个国家,牛结核病,牛结核病自由区或车厢 ;或 被保存在一个无牛结核病的牛群，并在30天的隔离期间成立之前收集原籍期间受到的负面结果结核菌素试验为牛结核病； 胚胎/卵的收集,处理和储存符合章的规定， 4.7 ，4。8和4。9。 ，作为有关.
第11。6。9。
进口鲜肉和肉制品,牛，水牛和木野牛的建议 进口国的兽医主管部门应当要求提交的国际兽医证书,证明整个寄售肉来自动物已宰前和宰后检查，如第二章所述 6。2。
第11。6。10。
进口牛奶和奶制品，牛，水牛和木野牛的建议 进口国兽医主管部门应当要求一个国际兽医证书证明货物的演示： 1. 2. 3.
来自已经动物在畜群无牛结核病； 遭到巴氏杀菌；
受到控制措施相结合，作为在食品法典奶及奶制品卫生实践规范具有同等的性能。
2011©世界动物卫生组织— 陆生动物卫生法典
因篇幅问题不能全部显示，请点此查看更多更全内容